Gene expression vector

Definition and Composition

Gene Engine (Genetic Engineering

Composition: Promotion, Terminal, Mark Gene, Purpose Gene < / p>

Also known as gene splicing technology and DNA recombination technology are the theoretical foundation of molecular genetics, with molecular biology and microbiology modern methods, and the genes of different sources are pre-designed. Hybrid DNA molecules are constructed in vitro, then introduce live cells to change the original genetic properties, obtain new varieties, and produce new products. Gene Engineering Technology provides a strong means of research on the structure and function of the gene.

DNA recombination

purpose gene gene and carrier combined

combined with theprocess of the purpose gene and carrier , actually different The process of re-combining the source of DNA. If a plasmid is used as a carrier,

first use a certain restriction enzyme to cut the plasmid, a gap is made, and the viscous end is exposed. The same restriction enzyme is then cut off, allowing it to produce the same viscous end (partially restriction endonuclease can be cut out of the flat end, with the same effect). The fragment of the cutting target gene is inserted into the incision of the plasmid. First, the base complementary pair is combined, and the two viscous ends have a hydrogen bond between the bases, and then add the appropriate amount of DNA linger enzyme, catalyze two DNA strands. A phosphate diodeyl bond is formed, thereby connecting adjacent deoxyribonucleic acids to form a recombinant DNA molecule. If a human insulin gene is bonded to the plasmid DNA molecule in E. coli by this method to form a recombinant DNA molecule (also called recombinant plasmid).

Technology Prospects

Science Propono, the 21st century is a genetic engineering century. The genetic engineering is intervention in the molecular level, to know it,

We first talk from the bioengineering: biological engineering is also known as biotechnology, is an application of modern life science principles and information and Chemical and other technologies, using activated cells or enzymes thereof to varying degrees of processing, providing a large number of comprehensive engineering techniques for useful products. The basis of bioengineering is modern life science, technical science and information science. The main products of bioengineering provide a large number of high-quality fermentation products, such as biochemical drugs, chemical raw materials, energy, biological prevention, and food and beverages, can also provide governance environments, extract metal, clinical diagnosis, gene therapy and improve crops. Social services such as varieties.

Biological engineering has 5 parts of genetic engineering, cell engineering, enzyme engineering, protein engineering and microbial engineering. The genetics are the transformation of biological genes, using biological production of special products you want. With the secret of DNA's internal structure and genetic mechanism, it is presented in front of people. The biologists are no longer only to explore, suggesting the secrets of biological genetic, but begins to jump, envisioned in the level of molecules. Genetic properties.

US Gilbert is the founder of the base arrangement analysis, and he takes the lead in supporting human genomenel if a genetic cryptos in a biological DNA is connected to another biological DNA. On the chain, reorganize DNA, you can not design a new genetic material and create new biological types according to human aspirations. This is completely different from the traditional practice of cultivating biological breeding offspring, and it is very similar to the engineering design of technical science, that is, according to human needs, the "gene" "gene" "gene" of this organism "gene", "construction", "Assembly" into new gene combination, creates new organisms. This is known as "genetic engineering", or "genetic engineering", or "genetic engineering"